Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers
نویسندگان
چکیده
منابع مشابه
Stretching DNA with optical tweezers.
Force-extension (F-x) relationships were measured for single molecules of DNA under a variety of buffer conditions, using an optical trapping interferometer modified to incorporate feedback control. One end of a single DNA molecule was fixed to a coverglass surface by means of a stalled RNA polymerase complex. The other end was linked to a microscopic bead, which was captured and held in an opt...
متن کاملImproved high-force magnetic tweezers for stretching and refolding of proteins and short DNA.
Although magnetic tweezers have many unique advantages in terms of specificity, throughput, and force stability, this tool has had limited application on short tethers because accurate measurement of force has been difficult for short tethers under large tension. Here, we report a method that allows us to apply magnetic tweezers to stretch short biomolecules with accurate force calibration over...
متن کاملMeasurement of the DNA Spring Constant Using Optical Tweezers
An optical trap was used to measure the relationship between restoring force and displacement of a 1.26 μm polystyrene microsphere attached to a 3.5 kilobase-pair DNA strand. Fitting to a wormlike chain model, the persistence length of the DNA molecule was measured as 53.0± 11.6 nm, and the contour length was measured as 713 ± 36 nm. The Hookean spring constant of DNA, at small displacements, w...
متن کاملOptical tweezers for force measurements on DNA in nanopores
We demonstrate the means to integrate two powerful and widely used single-molecule techniques, viz., optical tweezers and solid-state nanopores. This setup permits simultaneous spatial sampling and high-resolution force measurements of nucleic acids and proteins. First, we demonstrate the rapid spatial localization of nanopores using our custom-built inverted microscope and ionic current measur...
متن کاملAxial Optical Traps: A New Direction for Optical Tweezers.
Optical tweezers have revolutionized our understanding of the microscopic world. Axial optical tweezers, which apply force to a surface-tethered molecule by directly moving either the trap or the stage along the laser beam axis, offer several potential benefits when studying a range of novel biophysical phenomena. This geometry, although it is conceptually straightforward, suffers from aberrati...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2011
ISSN: 1940-087X
DOI: 10.3791/3405